Journal: Antioxidants

Article Title: Circuits Regulating Superoxide and Nitric Oxide Production and Neutralization in Different Cell Types: Expression of Participating Genes and Changes Induced by Ionizing Radiation

doi: 10.3390/antiox9080701

Figure Lengend Snippet: Cellular pathways influencing the production and neutralization of NO. The colored arrows show the direction of change (up/down regulation) of enzyme mRNA levels after the exposure of melanoma Me45 (green), K562 (red), and HCT116 (blue) cells to ionizing radiation.

Article Snippet: To study the changes in transcriptomes induced by irradiation, we used HCT116 (a human colon carcinoma cell line established from the primary colon carcinoma of an adult man, ATCC-CCL247), K562 (an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis, ATCC-CCL243), and Me45 (obtained from Dr M. Widel who established this cell line in the Center of Oncology in Gliwice from a lymph node metastasis of skin melanoma in 1997).

Techniques: Neutralization

Journal: Antioxidants

Article Title: Circuits Regulating Superoxide and Nitric Oxide Production and Neutralization in Different Cell Types: Expression of Participating Genes and Changes Induced by Ionizing Radiation

doi: 10.3390/antiox9080701

Figure Lengend Snippet: The tetrahydrobiopterin (BH4) pathway in NO and superoxide radical production and neutralization. The colored arrows show the direction of change of enzyme mRNA levels after the exposure of melanoma Me45 (green), K562 (red), and HCT116 (blue) cells to ionizing radiation.

Article Snippet: To study the changes in transcriptomes induced by irradiation, we used HCT116 (a human colon carcinoma cell line established from the primary colon carcinoma of an adult man, ATCC-CCL247), K562 (an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis, ATCC-CCL243), and Me45 (obtained from Dr M. Widel who established this cell line in the Center of Oncology in Gliwice from a lymph node metastasis of skin melanoma in 1997).

Techniques: Neutralization

Journal: Antioxidants

Article Title: Circuits Regulating Superoxide and Nitric Oxide Production and Neutralization in Different Cell Types: Expression of Participating Genes and Changes Induced by Ionizing Radiation

doi: 10.3390/antiox9080701

Figure Lengend Snippet: Response to ionizing radiation exposure in three cell lines: HCT116 (1), K562 (2), and Me45 (3) assayed at 1 h, 12 h, and 24 h after irradiation with 4 Gy of gamma rays (blue, red, and green bars, respectively). To better show the direction of changes, the results are presented as log2 of the fold change. Asterisks mark statistically significant fold changes.

Article Snippet: To study the changes in transcriptomes induced by irradiation, we used HCT116 (a human colon carcinoma cell line established from the primary colon carcinoma of an adult man, ATCC-CCL247), K562 (an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis, ATCC-CCL243), and Me45 (obtained from Dr M. Widel who established this cell line in the Center of Oncology in Gliwice from a lymph node metastasis of skin melanoma in 1997).

Techniques: Irradiation

Journal: Frontiers in Oncology

Article Title: Enhanced Cancer Therapy Using an Engineered Designer Cytokine Alone and in Combination With an Immune Checkpoint Inhibitor

doi: 10.3389/fonc.2022.812560

Figure Lengend Snippet: M7S (IL-24S) displays potent in vivo bystander activity. (A) 5X10 6 MeWo cells (melanoma cancer cell line) were injected subcutaneously into both flanks of nude mice. Once palpable tumors formed (~100 cc), Ad.5- null , Ad.5- mda-7 or Ad.5- M7S was administered intratumorally (3X in a week, 6 injections in total). Mice were sacrificed on day 21 after the first viral injections. Tumor weight was measured. Data presented as the average weight at day 14 from 5 mice from each group. (B) Like panel (A) , DU-145 cells (prostate cancer cell line) were implanted in nude mice and infected with the indicated control virus (Ad.5- null ) and therapeutic viruses (Ad.5- mda-7 or Ad.5- M7S ). Mice were sacrificed on day 21 after the first viral injections. Average tumor weight after 6 injections is presented. In both (A, B) , the “upper” and “lower” panels represent tumors with virus or without virus treatment, respectively. Error bars are S.D. from five mice. *Statistical significance (p < 0.05).

Article Snippet: RWPE-1, is a human papillomavirus 18 immortalized adult normal human prostate cell line obtained from the ATCC and maintained in keratinocyte serum-free medium (K-SFM) (Gibco) supplemented with 0.05 mg/ml BPE and 5 ng/ml EGF ( ).

Techniques: In Vivo, Activity Assay, Injection, Infection, Control, Virus

Journal: International Journal of Molecular Sciences

Article Title: Lamivudine, Doravirine, and Cabotegravir Downregulate the Expression of Human Endogenous Retroviruses (HERVs), Inhibit Cell Growth, and Reduce Invasive Capability in Melanoma Cell Lines

doi: 10.3390/ijms25031615

Figure Lengend Snippet: Concentration-dependent reduction of cell viability after antiretroviral treatments. Cell viability was assessed using the Sulforhodamine B (SRB) assay after 72 h of treatment with antiretroviral drugs in four human melanoma cell lines (SK-Mel-28, A375, FO-1, and MeWo), as well as in normal human epithelial melanocytes (NHEM). Dacarbazine, a medication commonly used in the treatment of melanoma, was employed for comparison. Data were acquired by calculating the mean ± S.D. of values from at least four independent experiments, which were then compared with the untreated control; * p < 0.05; ** p < 0.01.

Article Snippet: A375 (CRL-1619) and FO-1 (CRL-12177) melanoma cell lines and Normal Human Epithelial Melanocytes (NHEM, PCS-200-013) obtained from ATCC (Manassas, VA, USA) were cultured at 37 °C in a 5% CO 2 humidified atmosphere.

Techniques: Concentration Assay, Sulforhodamine B Assay, Comparison, Control

Journal: mSphere

Article Title: Recombinant chimeric horsepox virus (TNX-801) is attenuated relative to vaccinia virus strains in both in vitro and in vivo models

doi: 10.1128/msphere.00265-24

Figure Lengend Snippet: Multi-step growth kinetics of VACV-WR, VACV-IHD, VACV-Lis, VACV-NYCBH, TNX-801, and MVA in primary human cell lines from the dermal tract (melanocytes [ A ], keratinocytes [ B ], fibroblasts [ C ], and skeletal muscle cells [ D ]) at an MOI of 0.01. Samples were taken at 0, 12, 24, 48, 72, and 96. Triplicate six-wells were sampled at each time-point followed by three cycles of freeze/thaw/sonication and titrated on BSC-40 cells. The limit of detection is shown for the infectious (1.0 log 10 PFU/mL) and qPCR (3.3 log 10 genome copies/mL) assays with black dashed lines.

Article Snippet: Normal adult human primary cells were obtained from ATCC.

Techniques: Sonication

Journal: mSphere

Article Title: Recombinant chimeric horsepox virus (TNX-801) is attenuated relative to vaccinia virus strains in both in vitro and in vivo models

doi: 10.1128/msphere.00265-24

Figure Lengend Snippet: Multi-step growth kinetics of VACV-WR, VACV-IHD, VACV-Lis, VACV-NYCBH, TNX-801, and MVA in primary human cell lines from the respiratory tract (bronchial/tracheal epithelial cells [ A ], small airway epithelial cells [ B ], lung fibroblasts [ C ], and bronchial/tracheal smooth muscle [ D ]) at an MOI of 0.01. Samples were taken at 0, 12, 24, 48, 72, and 96 hpi. Triplicate six-wells were sampled at each time-point followed by three cycles of freeze/thaw/sonication and titrated on BSC-40 cells. The limit of detection is shown for the infectious (1.0 log 10 PFU/mL) and qPCR (3.3 log 10 genome copies/mL) assays with black dashed lines.

Article Snippet: Normal adult human primary cells were obtained from ATCC.

Techniques: Sonication